State assignment
State assignment of the Federal State Budgetary Institution ‘Research Institute of Experimental Medicine’ of the North-West Branch for 2014 and the planning period 2015-2016
State assignment of the Federal State Budgetary Scientific Institution ‘Institute of Experimental Medicine’ for 2015 and the planning period 2016-2017
State Assignment No. 007-01191-16 PR for 2016
State Assignment No. 007-01354-17-03 for 2017 and the planning period for 2018 and 2019
The plan of research work of the FSBSI ‘IEM’ for 2017-2019
State Assignment No. 007-00449-18-00 for 2018 and for the planning period of 2019 and 2020 of the 18th of January 2018.
The plan of research work of the FSBSI ‘IEM’ for 2018 and the planning period of 2019 and 2020
State assignment No. 075-00786-19-00 for 2019 and for the planning period 2020 and 2021 of the 29th of December, 2018.
Main scientific results
- Research of the fundamental foundations of life in health and disease
Integrative bases of brain activity in health and disease
— Revealed regional and temporal responses of CNS cells to systemic inflammation
— A hypothesis is put forward about the participation of calpains in dopaminergic transmission dysregulation.
— The expression of genes of neurotoxic and neuroprotective substances in the hypothalamus in traumatic brain injury is studied.
— The key role of the protein kinase CK2 in the mechanisms of signal transduction and synapse maturation is revealed.
Studying of the mechanisms of psychoemotional stress and resistance to it
— The death of neurons and suppression of neurogenesis in the progenitor zones of the brain after exposure to vital stress is revealed.
— disorders in the production of cortisol and prolactin is found in women who experienced vital stress.
— In the offspring of mothers who experienced traumatic stress, behavioral disturbances and spatio-temporal connections in the central nervous system is found.
— The activation of the system of orexin-containing and orexin-sensitive cells of the central nervous system and adrenal glands, an increase in the secretion of defensins under stress is revealed.
— It is established that the corrective effect in post-stress disorders is provided by the method of adaptive self-regulation based on the synchronization of cardiorespiratory relationships.
Studying of the genetic mechanisms of the formation of the pathological process
— The analysis of the occurrence of polymorphic alleles of the galanin gene in patients with different variants of the course of multiple sclerosis is carried out.
— Data on a new genetic marker of autoimmune lesions of the central nervous system is obtained.
— New polymorphisms of minisatellite repeats is found in patients with symptomatic epilepsy.
— The study of abnormal fibrillogenesis of model amyloidogenic proteins transthyretin and beta2-microglobulin is continued.
— 12 mutations and 6 polymorphisms in the LDL receptor gene in patients with familial hypercholesterolemia from Petrozavodsk are found.
— It is found that among the Slavs an epigenetically modified region of the bradykinin receptor gene can serve as a new biomarker of predisposition to coronary heart disease and myocardial infarction.
— Priority data on an increase in the expression of a number of genes in peripheral blood leukocytes in patients with epilepsy and Parkinson’s disease is obtained.
Dysregulatory pathology of organs and systems. Pathological integrations.
— An increase in the afterload of the heart reduces the severity of depression in the parameters of systemic circulation in myocardial ischemia.
— It is established for the first time that in the post-stress period the contractile activity of the jejunum significantly exceeds its initial level.
— The dependence of the function of cells of the immune and neuroendocrine systems on the activity of purinergic receptors is analyzed.
— The immunomodulatory effect of human lactoferrin and alpha-defensin of rat neutrophils under stress is discovered.
— Data on the role of ghrelin and orexin in the formation of alcohol dependence is obtained.
Studying of pathological anatomy and pathogenesis of socially significant human diseases
— A progressive form of unstable atherosclerotic plaque is identified For the first time. A classification of unstable and stable atherosclerotic plaques in humans is created.
— A close link is revealed between the infiltration of the vascular wall by immune-inflammatory cells and the processes of destabilization of the atherosclerotic plaque.
— New scientific data on the pathogenetic role of the anti-atherogenic protein apolipoprotein A-I and cholesterol transporter ABCA-1 in atherosclerosis is obtained.
— A method for innovative biochemical diagnostics of the threat of an acute coronary event (myocardial infarction) is developed.
— The concept of the pathogenetic role of immunoglobulin Fc-binding proteins of Streptococcus pyogenes as factors of initiation of immune inflammation in tissues during post-infectious complications (myocarditis, glomerulonephritis) is developed and experimentally substantiated.
Studying of the mechanisms and morphogenesis of the development of the nervous, endocrine, immune, lymphatic and visceral systems of a person in health and disease
— A previously unknown population of brain neural stem cells is characterized for the first time.
— The role of dopamine and NMDA receptor gene expression in the regulation of neuroplasticity in ontogenesis is studied.
— The ability of the components of pyogenic streptococcus to inhibit the functions of endothelial cells associated with angiogenesis is found.
Studying of the mechanisms and morphogenesis of the development of the nervous, endocrine, immune, lymphatic and visceral systems of a person in health and disease
— A previously unknown population of the brain’s own neural stem cells is characterized for the first time.
— The role of dopamine and NMDA receptor gene expression in the regulation of neuroplasticity in ontogenesis is studied.
— The ability of the components of pyogenic streptococcus to inhibit the functions of endothelial cells associated with angiogenesis is found.
II. Genomics, Proteomics, Post-genomic technologies, Metabolomics, Nanotechnology, Nanomedicine.
— Ideas about strictly maternal inheritance of the mitochondrial genome.
— Recombinant genetic engineering constructs have been created to study the mechanism of development of amyloidosis, including Alzheimer’s disease.
— A new unified method for the detection of 5-methylcytosine in chromosomes and nuclei of pre- and post-implantation embryos of mice and rats has been developed.
— The synthesis of acidic fullerene C60 derivatives as possible carriers of polar drugs in targeted delivery systems has been carried out.
— Efficient producers of receptors for human albumin and immunoglobulin G is obtained.
— Recombinant albumin- and immunoglobulin-binding polypeptides with high affinity for the main proteins of human blood serum is created.
— Chimeric protein-based polypeptides are developed for the most common strains of group B streptococci.
— A comprehensive study of their immunogenicity and specific protective efficacy is being carried out.
— An enzyme-linked immunosorbent assay is developed for the quantitative determination of RNP-3 defensin.
IV. Pharmacological correction of vital processes
— New low-toxicity adamantane-containing compounds are studied, capable of carrying out a combined block of AMPA-, NMDA-, and H-cholinergic receptors, possessing high neuroprotective, antihypoxic, anticonvulsant, and antiparkinsonian activity.
— The energy-stabilizing and antiarrhythmic effect of uridine derivatives is revealed in the model of acute myocardial ischemia with early postocclusive arrhythmias.
— It is established that activators and inhibitors of casein kinase-2 from the group of 1-alkyl-4,5-di (N-methylcarbamoyl) imidazoles are capable of modulating synaptic functions that are relevant for adaptation, learning, and the formation of a memory trace.
IX. Studying the patterns and mechanisms of the influence of the environment and work environment on the health status of the population
— It is shown for the first time that under the influence of ecotoxicants, DNA methylation in chromosomes is enhanced, and cleavage divisions of preimplantation mouse embryos are disrupted.
— Bisphenol A at low concentrations causes a paradoxical increase in cell proliferative activity, similar to that observed in carcinogenesis.
X. Basic and applied research on the problems of infectious epidemiology, medical microbiology, virology
